http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4055189-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e658c09c66d5a31f4987a0078881b54d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 |
| filingDate | 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe69d892781ce240c83432752e19949a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b18eb89408eb09bf3c9338122559e22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_133bc35af3419441b14ee307ef002ea3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cab27cb1fab19346f7773161cad98ce1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d4ce0ce5e0347cca37357908d3df47d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a05dd78c55483437199e40d93334d0d |
| publicationDate | 2022-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-4055189-A1 |
| titleOfInvention | Bivalent reverse primer |
| abstract | The present invention refers to a method directed to RT-qPCR reactions, preferably performed in a one or two-step approach combining the reverse transcription and subsequent PCR in a single tube and buffer, using a reverse transcriptase along with a DNA polymerase with mandatory 3' -> 5' exonuclease activity, which corrects miss-incorporated nucleotides. In particular, in this invention we present a One-step RT-PCR, preferably qPCR, method with a novel priming strategy that utilizes a novel bivalent reverse primer, wherein this primer is used for both, i) the generation of cDNA and ii) the completion of the subsequent amplification using that same cDNA as template. This bivalent reverse primer also allows end-tagging the amplicon(s) obtained so that they can be used in a variety of applications including, standard sequencing, next generation sequencing (NGS), gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research. |
| priorityDate | 2019-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 284.