| abstract |
Methods and compositions for the manufacture and use of a detection apparatus based on one or more native biological nanopores are provided. Uses include, but are not limited to, detection and sequencing of nucleic acids. The present invention relates generally to new methods and compositions for making protein-based nanopore sensors, more specifically, to methods of assembling native nanopore proteins in lipid nanodiscs, which are used as carriers to deliver the nanopore to a lipid membrane constituent of a sensor system, and to methods for the utilization thereof, particularly in nanopore-based nucleic acid sequencing methods. |