| abstract |
Disclosed are a transaminase mutant and use thereof. An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by a mutation of an amino acid sequence shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: G17V, L36P, Q40H, G69Y, H70T, L73A, V77G, V77S, V77T, A78I, Y130M, Y130V, Y130T, N132I, N132T, K141S, K142S, K142T, R143P, G144F, G144W, G144Y, E145D, E145S, E145G, K146R, L148A, L148I and the like. The transaminase mutant of the present disclosure obtains the catalytic activity on a carbonyl substrate. |