| abstract |
The invention relates to a method of producing a chromosome-free bacterial cell, 5 comprising: i) degrading native chromosomal DNA in a bacterial cell by expressing an endonuclease in the bacterial cell that is capable of recognition and double-stranded cutting of the native chromosomal DNA, wherein the expression of the endonuclease is under control of an inducible or repressible promoter; and ii) transforming the bacterial cell with recombinant nucleic acid encoding enzymes of one or more 10 biochemical energy pathways, or parts thereof, which provide the chromosome-free bacterial cell with energy. The invention further relates to associated compositions, chromosome-free bacterial cells and treatments. |