Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d9dbc2c0705931153800d351be59f09 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23L29-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y501-03 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L29-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 |
filingDate |
2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1770e5917d0657343cf17bd8895cf859 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_031daf1905c8c051c79b8bb1257640d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_842b832dc22371583d4a72bf6ea63e47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25e6fda6809bb83e06ca0472405229c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6267516093e36b8152a998f93f217a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_952d7dc8b540e88ccae05553b0df66b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8853c2b66de9bb82521e29f97aaa271d |
publicationDate |
2022-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-4026903-A1 |
titleOfInvention |
Allulose 3-epimerase mutant, engineered bacterium expressing same, and immobilized enzyme and immobilization method thereof |
abstract |
Provided are an allulose 3-epimerase mutant and a genetically engineered bacterium expressing the mutant. Further provided are an immobilized allulose 3-epimerase enzyme and an immobilization method thereof . A high-throughput screening method is used to obtain an allulose 3-epimerase mutant efficiently expressed in a fermentation process, which can catalyze efficient conversion of fructose to D-allulose, providing an efficient production path for key enzymes required in a D-allulose production process. Additionally, the allulose 3-epimerase is bonded to an immobilizing resin to prepare an immobilized allulose 3-epimerase enzyme. The immobilized enzyme can be applied to batch or continuous reactions to catalyze efficient conversion of fructose to D-allulose, improves the reuse rate and use life of the enzyme, can significantly reduce the production costs of D-allulose, and can be widely applied. |
priorityDate |
2020-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |