| abstract |
A method of testing genes for diagnosing thyroid nodules in an unspecified sample from biopsy (FNAB) material, from which RNA is isolated, then RNA upon transcription is synthesized into cDNA, followed by determination and normalization of gene expression. The material is a frozen or a freshly collected residual biopsy (washout needle) from washing the residue in the bevel of the needle, containing RNA at a concentration ranging from 4 ng/µl to 20 ng/µl, which is pre-amplified before transcription of total RNA at a number of cycles depending on RNA concentration. The length of the obtained gene amplicons ranges between 53base pairs and 134 base pairs. In addition, the expression of genes selected from transcription factors of the corresponding target gene is analyzed, and the normalization of expression of target genes is carried out under endogenous control of at least four normalizing genes, preferably six normalizing genes. The use of genes from the set including C3, CAMK2N1, DUSP5, EGR1, EMP2, FAM20A, FAXC, ITGA2, KCNQ3, MET, METTL7B, MPZL2, PSD3, SDC4, SLC26A4, SLPI, TM4SF1, TPO, SLC26A7, and TUSC3, for diagnosing of thyroid nodules in an ultra-small residual biopsy sample or full biopsy with RNA concentrations ranging from 4 ng/µl to 20 ng/µl. (10 claims) |