| abstract |
The present disclosure provides methods to recover repertoires of T cell receptors (TCRs). In some embodiments, TCR repertoires are recovered from non-viable samples. In some embodiments, libraries of TCRaβ pairs are created. In some embodiments, the methods disclosed are used for cancer immunotherapy or diagnostic purposes. Described herein, in some embodiments, are methods of identifying nucleotide sequences encoding T cell receptor a (TCRα)- and TCRβ-chains from a combinatorial library of nucleic acids. |