| abstract |
A method for preparing an analysis sample includes providing a sample containing a glycan, and performing an amidation reaction through contacting the sample with an amidation reaction solution having a pH of 7.7 or more, the amidation reaction solution containing at least one first compound which is to be reacted with a lactone included in the glycan, and which is selected from the group consisting of ammonia to be reacted with a lactone included in the glycan, primary amines having one or no carbon atom directly linked to a carbon atom linked to an amino group, hydrazine, hydrazine derivatives, hydroxylamine, and salts thereof, and alkalinizing agents to amidate the lactone, wherein when the alkalinizing agent is an amine, the alkalinizing agent is at least one amine selected from the group consisting of branched primary amines in which two or more carbon atoms are directly linked to a carbon atom linked to an amino group, secondary amines, and tertiary amines. |