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filingDate 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3995828-A1
titleOfInvention Novel functionalized hydrogel coatings of assay plates and uses thereof
abstract The present invention relates to the field of microplate assay-based analytical and diagnostic applications, in particular immunoaffinity assays, ELISA or ELISpot assays. More specifically, the present invention relates to functionalized hydrogel coatings of assay plates. The present invention provides a novel method of manufacturing a surface-bonded continuous layer of a copolymer that can be applied to assay plates used in analytical or diagnostic applications. The copolymer is characterized in that it comprises at least three different monomer units and includes at least one photo-reactive side group for a C, H-insertion cross-linking ["CHic"] reaction; and at least one click binding-reactive side group ["Click"] for (bio-) functionalizing of the copolymer layer. Furthermore, the present invention provides containers having such a novel surface-bonded continuous layer of a copolymer, as well as uses thereof for the selective attachment and/or capturing of biochemical reagents to the copolymer layer.
priorityDate 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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