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publicationDate 2022-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3990917-A1
titleOfInvention Analysis of oscillatory fluorescence from biological cells
abstract Methods and systems for analyzing oscillatory fluorescence representing an oscillatory ion flux associated with one or more biological cells. An illustrative method (50) of analysis may comprise detecting (54) fluorescence (91 ) from one or more biological cells (72) to produce a series of data points (120) describing an oscillation pattern (110). A series of slopes may be calculated (60) for the oscillation pattern (110). For example, a sliding window (122) may be used to define subsets (126) of the series of data points (120) from which the series of slopes are calculated. Peaks (118, 150a, 150b) of the oscillation pattern (110) may be identified (62) using the series of slopes. Primary peaks (118) and secondary peaks (150a, 150b), if any, in the oscillation pattern (110) may be identified and characterized by multiple measurements. An aspect of the secondary peaks (150a, 150b) may be determined. Appearance of secondary peaks (150a, 150b) may indicate a potential risk of cardiotoxicity or neurotoxicity.
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