Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e06a12f3d96af2dab618b5ff07019f65 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2020-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d039a28ab5ab758fe1d5aafc1b452f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_caf966463dcc50b385b29b6e0fcdea13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0142cc1e11fbe9985e3d63d2857de1b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_291fd65609eab1beeed7d430a9f2df15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64032c5df0bb5526c7aaf075d8a2e84e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c634342af3ed588f522ad0c3595a606a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06e96b8a825a4c1df43ea75d50e8ec6c |
publicationDate |
2022-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-3963104-A1 |
titleOfInvention |
Fast-forward sequencing by synthesis methods |
abstract |
Described herein are methods of generating a coupled sequencing read pair for a polynucleotide, and methods of analyzing the coupled sequencing read pair. The coupled sequencing read pair can be analyzed to detect polynucleotide variants, including at loci that are not directly sequenced within the coupled sequencing read pair. Other analytical methods can include using coupled sequencing read pairs to construct or validate a consensus sequence. The coupled sequencing read pair may be generated for a polynucleotide by generating sequencing data for a first region by extending a primer using labeled nucleotides; further extending the primer through a second region using nucleotides provided in a second region flow order, wherein primer extension through the second region is faster than primer extension through the first region; and generating sequencing data associated with a sequence of a third region of the polynucleotide by further extending the primer using labeled nucleotides. |
priorityDate |
2019-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |