http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3959342-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d275dfc66f330b15384758b6aba1dc3 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
filingDate | 2020-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3caab01a46c7da42908eb52d1817d67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8a11be42556821a3c9cfec9ef5175ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09749b5c450fdc655d390a6829ff9347 |
publicationDate | 2022-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-3959342-A1 |
titleOfInvention | Methods and kits for the enrichment and detection of dna and rna modifications and functional motifs |
abstract | Provided herein are methods for mapping modified nucleotide residues in nucleic acids. The methods include providing a nucleic acid sample in which non-target or target modified and unmodified nucleotide residues are converted to form of a different nucleotide (such a "C" being converted to "T"). Second strand synthesis is then performed on the converted nucleic acids using a set of anchored-base primers. Each primer in the set of anchored-base primers comprises one or more anchor bases at the 3' terminus that are complementary to the target nucleotide (e.g., "G" or "CpG"), and a sequence of nucleotides selected from a set of sequences that could be a fully or partially degenerate set of sequences. For example, the sequence could be 5'-XnG-3' and/or 5'-X(n-1)CG-3', wherein X is any base, and n=2 to 25. Double-stranded nucleic acid products can be analyzed, for example by amplification and high throughput sequencing. |
priorityDate | 2019-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.