| abstract |
The invention relates to a method of increasing the number of α2,3,-α2,6-disialylgalactose N -glycans on a glycoprotein by incubating an α2,3-sialylated glycoprotein with an α2,6-sialyltransferase and a sialic acid source. Also provided is a recombinant glycoprotein comprising at least one α2,3,-α2,6-disialylgalactose N -glycan. In a particular embodiment, the recombinant glycoprotein is alpha-1 antitrypsin (AAT). |