http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3943192-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_557fc96a66271e2b5a1132efe9f7fe00 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-089 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-161 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0673 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-168 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-165 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502792 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2018-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_645eb02a288c817eb905d9f10998bfcd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0358de5d99cd5c75f7319e9880223b5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5656c9d03629cc4940bb7b8d295c7de9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56559f438f79160469d45511f984ef48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea5e846824e78c36a5a6b3c32866d340 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4851c7e867f4c532050f5a48164407da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f8dae46d091927462806275d348f10f |
| publicationDate | 2022-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3943192-A1 |
| titleOfInvention | Microfluidic analytical device |
| abstract | A device for investigating a nucleic acid analyte characterised by comprising: • a first zone comprising an attachment site to which the analyte is attached; a first pathway for causing a first fluid medium to flow over the attachment site thereby allowing the nucleic acid to be progressively pyrophosphorolysed into its constituent nucleoside triphosphates; a second pathway for removing the nucleoside triphosphates from around the attachment site and a means for creating in the second pathway a second medium comprised of aqueous microdroplets in a water-immiscible carrier; • a microdroplet manipulation zone for manipulating the microdroplets using optically-mediated electrowetting comprised of: • a first composite wall comprised of • a first transparent substrate • a first transparent conductor layer on the substrate having a thickness in the range 70 to 250nm; • a photoactive layer activated by electromagnetic radiation in the wavelength range 400-1000nm on the conductor layer having a thickness in the range 300-1000nm and • a first dielectric layer on the conductor layer having a thickness in the range 120 to 160nm; • a second composite wall comprised of • a second substrate; • a second conductor layer on the substrate having a thickness in the range 70 to 250nm and • optionally a second dielectric layer on the conductor layer having a thickness in the range 120 to 160nm; • wherein the exposed surfaces of the first and second dielectric layers are disposed less than 10µm apart to define a microfluidic space adapted to contain microdroplets; • an A/C source to provide a voltage across the first and second composite walls connecting the first and second conductor layers; • a source of first electromagnetic radiation having an energy higher than the bandgap of the photoexcitable layer adapted to impinge on the photoactive layer to induce corresponding ephemeral first electrowetting locations on the surface of the first dielectric layer; • means for manipulating the points of impingement of the electromagnetic radiation on the photoactive layer so as to vary the disposition of the ephemeral electrowetting locations thereby creating at least one first electrowetting pathway along which the microdroplets may be caused to move; • an detection zone disposed downstream of the microdroplet manipulation zone or integral therewith and • a fluorescence or Raman-scattering detection system comprising a second source of electromagnetic radiation adapted to impinge on the microdroplets in the detection zone and a detector for detecting fluorescence or Raman-scattering emitted therefrom. |
| priorityDate | 2017-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 352.