http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3935184-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_620d8e0b5ce849d2ace9151c7b23d596 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
filingDate | 2020-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8aefbc87a8cd433e8d1f6d07e7595df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63b71d315ab5c43fcbc2a0ba7463701d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be7c50df3d4aebb567fc9972cbed1c8f |
publicationDate | 2022-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-3935184-A1 |
titleOfInvention | Compositions and methods of targeted nucleic acid enrichment by loop adapter protection and exonuclease digestion |
abstract | Compositions and methods for enriching DNA from any loci of interest are provided. Endonuclease(s) such as RNA-guided nucleases Cpf1/Cas9 can cut the flanking regions of the gene of interest in, for example genomic DNA, followed by ligation of loop adapters. The loop adapters protect the gene of interest for subsequent digestion with one or more exonucleases. Multiple genes of interest can be digested and ligated with loop adapters at the same time. The undigested target sequence can be further purified by, for example, gel extraction using, for example, a commercial DNA purification kit. The enriched DNA can be used for sequencing. |
priorityDate | 2019-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 206.