| abstract |
A CAR structure containing hypoxia-regulated promoter, an expression vector, a CAR-T cell and an application thereof, as well as a method for enhancing the ability of the CAR-T cell in secreting IFN-γ and/or IL-2 factors under an anaerobic environment and a method for improving the killing ability of the CAR-T cell under the anaerobic environment. The CAR structure contains the hypoxia-controllable promoter, and the hypoxia-controllable promoter is composed of a Hifla regulatory element and a mini-promoter. The number of repetitions of the Hifla regulatory element is 3-5. The CAR structure may be used for targeted therapy of tumors. |