| abstract |
Compositions and methods are provided for generating a clonal population of transfected eukaryotic cells derived from a single cell. The method includes transfecting a population of eukaryotic cells with a multi-cistronic nucleic acid vector followed by non-antibiotic selection and characterization of the selected cells. The multi-cistronic nucleic acid vector encodes a selection element which may be an autocrine protein, miRNA, and/or shRNA. |