| abstract |
This disclosure provides, among other things, a 5' adapter of the formula 3'*-X-(5'5')-Y-3', where: 3'* is a blocked 3' end, X is a synthetic sequence, (5'5') is an internal 5'-5' linkage, Y is an adapter sequence, and 3' is a hydroxylated 3' end. In use, sequence X hybridizes to sequence X' in a population of RNA molecules of formula R-X', which increases the efficiency of ligation of the 5' adapter to the nucleic acid molecules. |