| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_91b68d89dd92e5aa0158cf85c0fa1615 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H6-544 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate |
2019-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86f829f28dd49aa19bb1b087a409bcaa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c470f57a3d9b4d38aac58d521c683fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9e0c20ff27722eb24ed29f22398fcd1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97addaa68d29eaee0dcbd249dfed2fff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c5c26f5db339e44d8a6e0a52f3f2a3c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f37787762fbf9e90a815d8fe70832bd8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_783bd096765ded1f354ccb7145c3536f |
| publicationDate |
2021-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3816292-A1 |
| titleOfInvention |
Method of obtaining multi-leaf alfalfa material by means of mspalm1 artificial site-directed mutant |
| abstract |
Disclosed is a method for obtaining multileaflet Medicago sativa materials by means of MsPALM1 artificial site-directed mutants. The method comprises: selecting a target site from an exon region of a compound leaf developmental regulatory gene MsPALM1 of Medicago sativa and constructing a plant CRISPR/Cas9 editing recombinant vector MsCRISPR/Cas9::PALM1 and introducing the vector into Medicago sativa cells and regenerating into plants, cutting and repairing to cause a loss-of-function mutation in the MsPALM1 gene of Medicago sativa cells, and then screening the mutant plants by restriction endonuclease digestion and/or targeted deep sequencing of the target sites of the regenerated plantsgenome to obtain lines carrying four MsPALM1 allelic genes with simultaneous loss of function mutation. After phenotypic identification, it was confirmed that the compound leaves of the regenerated plants changed from three leaflets to five leaflets. The method can quickly obtain multileaflet Medicago sativa materials with a short breeding period and stable trait. |
| priorityDate |
2018-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |