| abstract |
Methods, instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one aspect a method of preparing a droplet containing a single pair of two biological entities comprises providing a first plurality of biological entities in a first fluid and providing a second plurality of biological entities in a second fluid and preparing a first droplet from said first fluid and preparing a second droplet from said second fluid. The method further comprises determining whether the first droplet contains a single entity of the first plurality of biological entities, and whether the second droplet contains a single entity of the second plurality of biological entities, and fusing the first droplet and the second droplet which have been determined to contain a said single entity, respectively. |