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filingDate 2019-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3799956-A1
titleOfInvention Electrophoretic enrichment of analytes in droplets
abstract In a method for increasing a concentration of an analyte in a sample liquid, a stream of sample droplets separated by a separating fluid is created. The droplets are sequentially transported through a microfluidic channel (21). A DC electric field is applied across the microfluidic channel to cause a concentration gradient of the analyte within each sample droplet while the sample droplet moves through the microfluidic channel. Each sample droplet is then split into at least two daughter droplets, one of the daughter droplets having a higher concentration of the analyte than the other daughter droplet. The analyte can comprise a molecular species, including a peptide, protein or nucleic acid, and the molecular species can be directly dispersed in the sample liquid without being attached to macroscopic particles.
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