http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3797171-A1

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filingDate 2019-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d5a3bec72e78578b1adc5cf13711c12
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publicationDate 2021-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3797171-A1
titleOfInvention Single cell mapping and transcriptome analysis
abstract Methods of tagging cells with unique oligonucleotide "zipcode" constructs are provided. By these methods and associated compositions, cells in a multicellular structure such as a tissue section can be tagged with a construct, the unique composition of which is associated with the cells position in the multicellular structure. Subsequently, the multicellular structure can be dissociated into single cells and a single cell transcriptome analysis performed, as well as other types of single cell analyses. By preserving positional information in the analyzed single cells, biological processes within the tissue can be mapped. By these methods, the effects of the local environment surrounding a cell on its state and various functions can be elucidated, and intra-tissue processes can be mapped and observed. Likewise, coordinated actions by multiple cells within a tissue can be mapped and tracked over time.
priorityDate 2018-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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