Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e70c4209b795d679aba9b3cb43115d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1414f409b67cf2ee73ea01ca71c27f7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f9ca02c52dbfdac277481e39bde93cfd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74f35a038deb20e02f08206b9f6f59a0 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 |
filingDate |
2019-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b28af3143e1082deb42811542b19ffc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e490f00f5dd2bcb243471bba7846a14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_376b60c9e3db84e0d2d214ebb58a305e |
publicationDate |
2021-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-3788378-A1 |
titleOfInvention |
Qualitative analysis of proteins |
abstract |
The present invention relates to a method for qualitative analysis of a sample protein, which method comprises the steps of providing a water swollen gel column bed comprising bound protease and a sample protein in liquid buffer, digesting the sample protein into polypeptides by contact with the gel bed and subjecting the polypeptides to mass spectrometry (MS). The method is advantageously performed using back and forth flow of the sample protein including two or more repeats, and may be performed at a temperature of less than about 37°C, such as a temperature of less than about 30°C.The invention also includes an automated method as well as a device and a kit for performing mass-based analysis of proteins with higher speed than the prior art while maintaining conditions that are tolerable to the protease. |
priorityDate |
2018-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |