| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd7c0ee90e9a61b4c9d6ca5a253180ff |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15051
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-16043
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-16051 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-02 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate |
2019-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_daf5e4e22641c0a9b602a7fd2bb5d6bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81e258e42ac39c8741d5bb27c8100012
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02c44b7b30ff57684402d6dc43dd5758
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ff043e15a17690f605b94e75ced1fca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5436fd48c40cdf23bb610ab5f44e5273
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41d5d7d34cb8b52937c9c50fb9b5f844 |
| publicationDate |
2021-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3778880-A1 |
| titleOfInvention |
Method for large-scale production of lentivirus by using gmp-level serum-free suspension cells |
| abstract |
Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaging cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaging cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaging cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution. |
| priorityDate |
2018-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |