http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3775850-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7aea4de554faab6ae9fe0540f039313a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8c3c03868c3cbb1941929d9d8bc3f664 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-365 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-16 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f6fe3df1a6d2b591b1dccf026d84741 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6936264a7e4e2f3cb09699edb16185e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb70642718c1df850e525554202dd292 |
| publicationDate | 2021-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3775850-A1 |
| titleOfInvention | Scattering-assisted super-localization microscopy method and relative apparatus |
| abstract | Fluorescence microscopy method comprising the following steps: A. illuminating a scattering sample including one or more fluorescent emitters with a coherent excitation light beam (5) having a wavefront with a first initial configuration C 1 for exciting said one or more fluorescent emitters comprised in the scattering sample; B. acquiring a first initial image F 1 corresponding to a background image F 1 =F 8 of the scattering sample, through a pixel imaging unit (45), obtaining a speckle grain image; C. selecting M target pixels P N , with N = 1,…,M, of coordinates [X N , Z N ] belonging to an area of the first initial image F 1 having fluorescence signals of at least one fluorescent emitters, each one of the M target pixels P N having a first initial intensity I PN 1 ; D. optimising the first initial configuration of the wavefront for each one of the M target pixels P N , for decreasing the speckle grain size of the first initial image F 1 and obtaining a final image F fin (I PN max ) of the scattering sample with a local maximum I PN max of the fluorescence signal corresponding to a maximum intensity of the target pixels P N ; E. subtracting the first initial image F B of background, obtained at step B, from the final image F fin (I PN max ), obtained at step D, for each one of the M target pixels P N , obtaining an image F speckle ( PN ) having only fluorescent signals coming from a speckle grain located at pixel [X N , Z N ] with intensity I PN speckle , for each one of the M target pixels P N ; F. fitting the image F speckle ( PN ), obtained at step E, with a Gaussian function with free center coordinates, for each one of the M target pixels P N , thereby obtaining coordinates (X N , Z N ) and intensity I N in a xy plane; G. generating an image F N containing a Gaussian distribution centered at coordinates (X N , Z N ) and with intensity I N , obtained at step F, and with a waist equal to an average size S of speckle grain of the scattering sample, for each one of the M target pixels P N ; H. generating a final image of the sample by summing the M images F N obtained at step G, for each one of the M target pixels P N ; |
| priorityDate | 2018-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID65182 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226432888 |
Total number of triples: 19.