| abstract |
Described herein are variants of alpha-hemolysin having at least one mutation, such as a mutation to a positive charge. In certain examples, the mutation is selected from OK, A1 K, A1 R, D2N, S3K, D4K, D4N, K8R, N47K, E70K, S106K, E1 1 1 N, 127-131 G, D128K, K147N, V149K, E287R, M298A, or combinations thereof in the mature, wild-type alpha-hemolysin amino acid sequence. Also provided are compositions including the variants of alpha-hemolysin, nanopore assemblies including the alpha-hemolysin variants, and methods of sequencing nucleic acids incorporating the same. |