| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_35c66e8c9cb40e017b654c03f23e3cb2 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-067
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8813
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-466
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-368
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-4061 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-92
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-405
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-7266
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-4055
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate |
2019-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28ab572e507e306b5363563bf3e35aca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a1c1a53c0063ddb1ae9da77793b1163
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b73dc04a0b125f47b86cad5d57e3ccc |
| publicationDate |
2020-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3749952-A2 |
| titleOfInvention |
A method of processing a biological sample |
| abstract |
A method of processing of a biological sample containing multiple metabolites is described The method comprising the steps of pre-treating the biological sample with a metabolite extraction solvent to provide a pre-treated sample, separating a first aliquot of the pretreated sample by reverse phase liquid chromatography (RPLC) to provide a first eluent containing resolved hydrophobic metabolites, and separating a second aliquot of the pre- treated sample by hydrophilic interaction liquid interaction chromatography (HILIC) to provide a second eluent containing resolved hydrophilic metabolites. The first and second eluents are assayed using targeted tandem mass spectroscopy operated in multiple reaction monitoring mode. Each liquid chromatography step(LC) is directly hyphenated with the tandem mass spectrometry (MS/MS) into a single LC-MS/MS analysis. The extraction solvent typically comprises methanol, isopropanol and an acetate buffer. |
| priorityDate |
2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |