http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3743522-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b0a17343b9dfb09ff9dfce16bee9c0d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A90-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16H10-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2019-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccaa8848412164094b70f6fd7b7a0c80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cbdd608d0673503c69022987bc70b46 |
| publicationDate | 2020-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3743522-A1 |
| titleOfInvention | Method for determining microorganism concentration |
| abstract | The present invention provides a method of preparing a suspension of intact microorganisms from a sample containing microorganisms and mammalian cells,comprising contacting the sample with a buffer solution with a pH of at least pH 6 and less than pH 9, a detergent and one or more proteases to allow lysis of the mammalian cells; filtering the mixture through a filter suitable for retaining microorganisms to remove the lysed mammalian cells; resuspending the microorganisms retained by the filter in a liquid to provide a suspension comprising the recovered intact microorganisms; and determining the concentration of microorganisms in the suspension by:i.heating and/or contacting an aliquot of the suspension with an alcohol;ii.optionally diluting one or more aliquots of the suspension to provide one or more diluted aliquots before, during and/or after step (i);iii.contacting at least a portion of an aliquot of step (i) or (ii) with a single fluorescent stain capable of binding to DNA; iv.imaging the mixture of step (iii) at the emission wavelength of the fluorescent stain and determining an image analysis value for the number of objects corresponding to microorganisms in the imaged mixture; and v.comparing the image analysis value to a pre- determined calibration curve, thereby to determine the concentration of microorganisms in the suspension. |
| priorityDate | 2018-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 450.