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filingDate 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3734282-A1
titleOfInvention Apparatus, reagent kit, and method for detecting misfolded protein
abstract The invention discloses a device, a kit and a method for determining whether a biological sample contains misfolded protein or misfolded protein aggregates. The detection device comprises a testing component and a sample applicator, the testing component comprises one or more microporous membranes, and the sample applicator comprises one or more capillary tubes. The kit comprises a dye that is capable of binding to misfolded proteins and microporous membranes. During the detection, the sample to be tested is mixed with the dye to form a mixture. The mixture is taken up by a capillary tube. The liquid outlet of the capillary tube is then place in close contact with the surface of a microporous membrane, allowing the mixture to be drawn from the capillary tube into the microporous membrane. The presence or absence of misfolded proteins in the test sample is determined based on the diffusion of the dye in the membrane, as observed by naked eyes or using an instrument.
priorityDate 2017-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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