| abstract |
Embodiments of the disclosure relate to methods and compositions for inducing maturation of cardiomyocytes. In some embodiments, the cardiomyocytes are derived from stem cells, in vitro. In some embodiments, the compositions and methods induce maturation by inducing overexpression of a Let7i microRNA (miRNA), overexpression of miR-452, reduced expression of miR-122, and/or reduced expression of miR-200a in the cardiomyocyte. In other embodiments, the disclosure relates to methods for treating conditions characterized by mitochondrial dysfunction, such as fatty acid oxidations disorders. In other embodiments, the disclosure relates to methods of screening for compounds that affect heart muscle function. In yet other embodiments, the disclosure relates to methods for detecting or monitoring mitochondrial dysfunction in a cell by detecting or monitoring the cardiolipin profile of the cell. |