| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f33fb172331a8b7b60d4800567390618
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e22608e1ca3db0f92b706c4ddf6d4385 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H6-827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8287
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8289
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-82 |
| filingDate |
2019-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3304ffeaae1ddb71b43f36e423294bf5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a9937d8bfc8dd6bb75bdff1720584e8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e309905fadc74efa813b7c79c2cf4976
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4c76fd47bb682b17e743a2032ce30ed |
| publicationDate |
2020-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3719132-A1 |
| titleOfInvention |
Method for overcoming self-incompatibility of diploid potatoes |
| abstract |
Disclosed is a method for overcoming self-incompatibility of diploid potatoes, which comprises: (1) selecting a target fragment; (2) constructing a gene-targeting recombinant vector; (3) achieving a loss-of-function mutation of the intracellular S-RNase gene; (4) regenerating a plurality of potato plants; (5) specifically amplifying a DNA segment of the S-RNase gene in a regenerated plant, wherein the DNA segment containing the target fragment; (6) selecting a regenerated plant in which the S-RNase gene is edited; (7) further screening the selected gene-edited plant for a diploid gene-edited plant line; (8) propagating and planting the selected gene-edited plant line, and identifying the self-compatible phenotype at the flowering stage; and (9) sequencing the gene amplification products of the harvested offspring of the self-compatible plant, and detecting the inheritance and isolation of the offspring in which the target gene is edited. The advantage of the present invention lies in that a simple, accurate and efficient method for overcoming the self-incompatibility of diploid potatoes is found. |
| priorityDate |
2018-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |