| abstract |
The present invention aims to provide a method for efficiently producing, from pluripotent stem cells, a cell mass containing a neural cell or neural tissue, and nonneural epithelial tissue. A method for producing a cell mass containing 1) neural cells or neural tissue and 2) nonneural epithelial tissue, including the following steps (1) and (2):(1) a first step of suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,(2) a second step of suspension-culturing the aggregate obtained in the first step in the presence of a BMP signal transduction pathway activating substance, thereby obtaining a cell mass comprising 1) neural cells or neural tissue and 2) nonneural epithelial tissue. |