| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a910d33c239980f3a705e312c5bad6a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-21004 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate |
2018-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc27dc938734e96e961db3c7558b9fac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a66995823711c5252835dd4b9eb02d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfc0ae51bfab8282f0b3205ab292698e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cb836eddab8cd0afc4ed7e2d7b40092
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2325c2ed1a97ed0598eb5fba733f8636 |
| publicationDate |
2020-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3710583-A1 |
| titleOfInvention |
Compositions and methods for improving the efficacy of cas9-based knock-in strategies |
| abstract |
The present disclosure provides a non-naturally occurring CRISPR-Cas system comprising: a Cas9 effector protein capable of generating cohesive ends (stiCas9), and a guide polynucleotide that forms a complex with the stiCas9 and comprising a guide sequence, wherein the guide sequence hybridizes with a target sequence in a eukaryotic cell but does not hybridize to a sequence in a bacterial cell, and wherein the complex does not occur in nature. The present disclosure also provides a method of introducing a sequence of interest into a chromosome of a cell. Finally, the present disclosure provides for a method of modifying one or more nucleotides using seamless mutagenesis. |
| priorityDate |
2017-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |