| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50eb752940fa4aa36756a6bc9f6f3504 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2015-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7633b095988a0af853250391cffec9af
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e60c3b63b3608c9868029d5ae0160326
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b89e317cba07131e6eb83eb1090ed672
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c34800753595353e2e6938c1405769a5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5138de28d52db94bfa8d0a11f3bef14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_578f41378abf7ab3f5289619954b9c69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5297213fcdef57cdd287b76ca1bc2780
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bdecf66a3b501d0acf742e01a6b9f773
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b0e65f6722dab22c8233aa56f25f2bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df25ebad11b4626dbe8036244088360b |
| publicationDate |
2020-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3708663-A1 |
| titleOfInvention |
Nuclease-mediated dna assembly |
| abstract |
Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends. |
| priorityDate |
2014-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |