| abstract |
The object of the invention is a method for decontamination of environment polluted with sulfuric esters, including surface active agents containing an ester bond of sulfur esters using a microorganism expressing alkylsulfatase or isolated bacterial alkylsulfatase, wherein the alkylsulfatase comprises an amino acid sequence encoded by a nucleotide sequence comprising sequence set forth in SEQ ID NO: 3 or 4 or at least 95% identical with it, in particular the alkylsulfatase comprises the amino acid sequence set forth in SEQ ID NO: 1 or 2, or a sequence at least 95% identical to it; wherein said microorganism, its lysate or enzyme having alkylsulfatase activity isolated from microorganism is introduced into the environment to be purified. The invention also relates to new bacterial strains Pseudomonas jessenii AP3_16 B/00139 and Pseudomonas laurylsulfatovorans AP3_22, a method and use thereof. The invention further relates to the novel alkylsulfatase coding proteins, a nucleic acid construct, an expression vector, a host cell, a method for the production of novel bacterial strains, a method for producing an alkylsulfatase protein, a composition comprising said protein, the strain or lysate of the bacterial strain as well as a method for bioremediation of an environment contaminated with xenobiotics using thereof. |