| abstract |
A method for producing a hydrolyzed pectic polysaccharide isolate that is enriched in rhamnogalacturonan-1, said method comprising the steps of: • providing a pectin-rich substrate that has been obtained from plant material without using organic solvent, said pectin-rich substrate containing at least 3% by weight dry matter of pectic polysaccharides; subjecting the pectin-rich substrate to enzymatic treatment to partially hydrolyze the pectic polysaccharides, said enzymatic treatment comprising the use of one or more pectinases selected from pectin lyase (EC 4.2.2.10), pectate lyase (EC 4.2. 2.2), rhamnogalacturonan galacturonohydrolase (EC 3.2.1.173), endopolygalacturonase (EC 3.2.1.15), exopolygalacturonase (EC 3.2.1.67 and EC 3.2.1.82); subjecting the partially hydrolysed pectic polysaccharides to ultrafiltration using an ultrafiltration membrane having a retention threshold of molecules in the range of 5 to 100 kDa; and • recover the ultrafiltration retentate. The present invention further relates to the hydrolyzed pectic polysaccharide isolate obtained by the present process and to a process for preparing a product selected from a nutritional formulation, a food product, a food supplement, a beverage or a pharmaceutical product, said method of adding the aforementioned hydrolysed pectic polysaccharide isolate. |