Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18ba3a1a4c6973da04eb4b2d18a002f6 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-121 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
filingDate |
2015-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa2993829510efc7120e7078a37ae57e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a237895fdd8179f6820d420ca2e0fb7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c24524fea7b2eda81bc4761d08b5a63a |
publicationDate |
2020-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-3686278-A1 |
titleOfInvention |
Genetic targeting in non-conventional yeast using an rna-guided endonuclease |
abstract |
Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5'-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5'-cap. Further disclosed are methods of genetic targeting in non-conventional yeast. |
priorityDate |
2014-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |