| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1fbefd12d7aa2f22e8527d42ad2dcc55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46a62c3451ead80714a6a0decddfe17b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b69cd7b92e37156b098adc0bd40a0933
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6b6017ace434d2cacc3752969f14080a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2560-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-7028
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-912 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5091
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57488
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B40-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 |
| filingDate |
2018-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51deef789a5ce81ccda7321941c877c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2f924dacfd42a92abaf68ff8ab03b84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fe98fa8597619891b86b7e9110b1d28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba40802463d2cef0cd30b3a7d2edd82b |
| publicationDate |
2020-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3682244-A1 |
| titleOfInvention |
Method and system for identifying membrane proteins on extracellular vesicles |
| abstract |
The invention relates to a method of isolating extracellular vesicles and identifying membrane proteins therefrom. The method comprises the use of plasma and / or human serum; separating extracellular lipoproteins and vesicles from plasma and / or human serum by a density gradient preparation; collection of extracellular vesicles from separated lipoproteins and extracellular vesicles; isolation and purification of the extracellular vesicles collected using an exclusion-diffusion chromatography; treating extracellular vesicles isolated and purified with an aqueous solution to obtain extracellular vesicle membranes, the aqueous solution having a pH of between 9 and 14; the addition of salt at a concentration of between 0.5 and 2.0 M in the aqueous solution; isolation of treated extracellular vesicle membranes and identification of proteins on isolated membranes by mass spectrometry. |
| priorityDate |
2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |