| abstract |
The present invention relates to the application of biosynthetic engineering for the heterologous expression of a gene cluster for the biosynthesis of tetracycline compounds, notably chelocardin and its analogues. More particularly, the present invention p ertains to a gene cluster encoding polypeptides involved in tetracycline biosynthesis, which gene cluster is suitable for heterologous expression of the biosynthetic pathway in a host cell. The present invention further pertains to DNA construct s comprising the gene cluster, to recombinant heterologous host cell s comprising the gene cluster or the DNA construct, to p rocess es for the biosynthetic production of a tetracycline compound employing such recombinant host cells, and to tetracycline compou nd s thereby produced. The present invention also pertains to fusion proteins which are useful in the production of tetracycline compounds. |