| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate |
2018-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_356ec39ba440c82ee0fea2311af6e51b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03376dfd995a36fe6b6daf0097487760
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_829a2f17add5bb12b33ea02052219b76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_452af5f2ca9ed5228198ab18eedb7d56 |
| publicationDate |
2020-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3677673-A1 |
| titleOfInvention |
Glutamate dehydrogenase mutant and use thereof in preparing l-glufosinate |
| abstract |
The present invention discloses glutamate dehydrogenase mutants and their application in preparation of L-phosphinothricin. The amino acid sequences of the glutamate dehydrogenase mutants are as shown in SEQ ID NO.1-9, 11, 13, 15, 17-19 and 22. By means of molecular engineering, mutating the specific alanine in glutamate dehydrogenase substrate-binding pocket into glycine and/or mutating the specific valine in glutamate dehydrogenase substrate-binding pocket into alanine, the present invention has obtained NADPH-specific glutamate dehydrogenase mutants with high enzyme activity in catalyzing the substrate 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid or its salt for L-phosphinothricin preparation or NADH-specific glutamate dehydrogenase mutants with catalytic activity toward PPO; this has significantly improved substrate conversion, and increased the product concentration of the L-phosphinothricin preparation process. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112280760-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111876396-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111876396-B |
| priorityDate |
2018-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |