| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b9df18b06c0d3a16c8aa0e56310fa5b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-025
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-635
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate |
2018-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f640bedcaac35b83b7dd42f033f7ecec
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19243110471d108fddec472040b4f24c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_061149814a041a3da5c61dc04092f5eb |
| publicationDate |
2020-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3677668-A1 |
| titleOfInvention |
Gene construct and biosensor for the rapid detection of ahl molecules and the pathogenic bacteria that produce same |
| abstract |
The invention relates to a gene construct for detecting the presence of microorganisms that produce chemical molecules of the Acyl-homoserine lactone (AHL) type, comprising a first expression cassette that includes a copper-inducible promoter operably linked to the gene encoding the RhIR protein and, downstream of said first expression cassette, a second expression cassette including a promoter that is induced by the AHL-RhIR complex, said promoter being operably linked to a gene encoding a reporter protein. The invention also includes a genetically modified biosensor cell to detect the presence of microorganisms comprising said gene construct, as well as the method of detecting the presence of microorganisms that produce AHL-type chemical molecules by immobilizing the biosensor in an organic matrix, generating a surface that can be used to expose a sample of a fluid such as air or a liquid medium, and thus detect these molecules mediated by the biosensor cell. |
| priorityDate |
2017-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |