http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3677336-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a2080e3d36502de777c9cd71ff1c152e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502761 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0487 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0838 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0829 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502715 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-50273 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 |
| filingDate | 2008-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17e824b4c64f24142ac0bc0e59808d47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_213a34c66663107bd2a0ab4f7c91ef88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d4fef6a232f3c27b9dcd7a79d7d40fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31456dc74c8629c288a9c657833004a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9f1c490c1abc76b163a2659567cc431 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3e1f46b37c88729f4421125eb9a1b27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ba632d1bdd321db86360576152a9a39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6402b15451d8c3327dfd7925a86336ab |
| publicationDate | 2020-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3677336-A1 |
| titleOfInvention | Microfluidic method and system for enzyme inhibition activity screening |
| abstract | Kits and systems for screening a compound for enzyme inhibition activity using a microfluidic device 110, the microfluidic device having at least one microchannel 102 and a capillary element 112, the capillary element operably connected to and in fluid communication with the microchannel, the kit comprising a first multiwell plate 150 having a plurality of enzymes disposed within a first plurality of wells; a second multiwell plate 160 having a plurality of enzyme substrates disposed within a second plurality of wells, each one of the second plurality of wells corresponding to one of the first plurality of wells; a phosphate source 162 disposed within each well of the second plate, the phosphate source to be disposed in each of the well at a predetermined phosphate source concentration; a cofactor 164 disposed within each well of the second plate, the cofactor disposed in each well at a predetermined cofactor concentration; wherein the enzyme within each well of the first plate is selected to react with the enzyme substrate, the phosphate source, and the cofactor in the corresponding well of the second plate when the compound is not present; and an instruction set stored on a computer readable medium, the instruction set comprising instructions for controlling dipping of capillary elements into the first plurality of wells; wherein the microfluidic device has a first reservoir and a second reservoir, the first reservoir having a depth greater than the depth of the second reservoir, the first reservoir having a larger volume relative to the volume flow rate of the at least one microchannel such that a sample is flowable into the at least one microchannel of the microfluidic device via the capillary element of the microfluidic device by a hydrostatic pressure differential created by the depth differential between the first reservoir and the second reservoir. |
| priorityDate | 2007-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 152.