http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3670664-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efb35e154fff5d3177ae8520d41046d5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2014-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d103942df7934b610cb2e06ded35cda2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15d8291fce3b676be42dc4eec905e043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_752423dcf2a72d8c27233ad509764e37 |
| publicationDate | 2020-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3670664-A1 |
| titleOfInvention | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| abstract | There is described a method of processing a specimen that includes a clinical sample disposed in a liquid-based cytology preservative that comprises formaldehyde, the method comprising the steps of: (a) combining the specimen with proteinase K and formaldehyde scavenger to create a reaction mixture, wherein the formaldehyde scavenger is succinic acid dihydrazide; (b) incubating the reaction mixture at between 65 and 100 °C for a period of time sufficient to reverse chemical modifications of nucleic acid that may be contained in the specimen by formaldehyde in the liquid-based cytology preservative; (c) isolating a nucleic acid from the reaction mixture after the incubating step; and (d) performing anin vitroamplification reaction using the nucleic acid from the isolating step as templates. |
| priorityDate | 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.