http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3668999-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9288fab0395161486a1e9c3c2548a936 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6818 |
| filingDate | 2018-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05c6d2c3b2d7310ab9f15813db34f8ec |
| publicationDate | 2020-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3668999-A1 |
| titleOfInvention | Methods and kits for detection of nucleic acid molecules |
| abstract | The invention relates to a method for detecting one or more polynucleotide amplification products, the method comprising, in one embodiment, the steps of: a) providing one or more oligonucleotide primer groups, each group comprising i) a forward primer comprising a fluorescence cassette specific portion and a downstream target specific portion 3 "; and ii) one or more target specific primers; b) providing one or more fluorescent pairs extinguished, each wherein said pair is characterized by: i) a first cassette oligonucleotide labeled with a fluorescent moiety and having a sequence that is capable of hybridization with the complement of the fluorescence cassette specific portion of the primer before a group of oligonucleotide primers, and ii) a second cassette oligonucleotide labeled with a quencher moiety; c) initiating an amplification reaction; isothermal polynucleotide in the presence of a strand displacement DNA polymerase, thereby generating a sequence complementary to at least a portion of the relevant forward primer, such that the second relevant cassette oligonucleotide is less able to hybridize to the first relevant cassette oligonucleotide, whereby a signal is generated; and d) detecting the signal. |
| priorityDate | 2017-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.