http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3655528-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e0bf0928b608797b70bbc2b91c7e52e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2018-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d0d98bd92d579f16ed5ca21b5bab177 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61ce9b8e3035f7e1131dd28ba8f8851d |
| publicationDate | 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3655528-A1 |
| titleOfInvention | Method of transforming bacterial cells |
| abstract | The present invention relates to production of DNA methyltransferase in a recombinant host cell, DNA methyltransferase methylates DNA, resulting in DNA containing 5-methylcytosine within the GCNGC recognition sequence and DNA methyltransferase comprises less than 35 amino acid residues between the amino acid residue 72 and the amino acid residue 106 according to the numbering of SEQ ID NO: 33. In addition, the present invention relates to the use of such DNA methyltransferase for the production of bacterial transformants, comprising the steps of: a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA (b) transferring the DNA methylated from the first bacterial host cell into a second bacterial host cell, the second bacterial host cell comprising a restriction endonuclease capable of degrading DNA but unable to degrade methylated DNA. |
| priorityDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1412.