http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3650559-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53c911e75ea09dd4c2705db5306dae77 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-531 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa8f687f73219288c60b637409799831 |
| publicationDate | 2020-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3650559-A1 |
| titleOfInvention | Direct rna nanopore sequencing with help of a stem-loop polynucleotide |
| abstract | The present invention relates to a method of characterizing a target RNA polynucleotide comprising (i) providing an RNA polynucleotide; (ii) modifying said RNA polynucleotide by annealing and ligating a polynucleotide comprising a 3' terminal random multimer segment and having a stem-loop form; (iii) optionally performing a reverse transcription of the RNA polynucleotide; (iv)cleaving the stem-loop segment of said annealed polynucleotide to yield a 3' A overhang; (v) connecting an adaptor polynucleotide complex associated with an RNA translocase enzyme and at least one cholesterol tether segment to the polynucleotide obtained in step (iv); (vi) contacting the modified RNA polynucleotide obtained in step (v) with a transmembrane pore such that the RNA translocase controls the movement of the RNA polynucleotide through the transmembrane pore and the cholesterol tether anchors the RNA polynucleotide in the vicinity of the transmembrane pore; and (vii) taking one or more measurements during the movement of the RNA polynucleotide through said transmembrane pore, wherein the measurements are indicative of one or more characteristics of the RNA polynucleotide, thereby characterizing the target RNA polynucleotide. Also provided is a kit for characterizing a target RNA polynucleotide, as well as a use of a polynucleotide comprising a 3' terminal random multimer segment and having a stem-loop form for the modification of an RNA polynucleotide. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022243691-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023154159-A1 |
| priorityDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.