abstract |
The present invention relates to a surfactant-containing wash buffer for use in cation exchange and affinity chromatography for purifying proteins of interest from protein aggregates and for removing and / or inactivating viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves the elimination of virus from the preparation, while also reducing host cell protein levels and protein aggregates. After affinity or cation exchange with the wash buffer, the protein of interest can be further purified by further chromatography and filtration. |