http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3641935-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b5d47c4f1d77d82de7ce1f64ba985b3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-161 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-168 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0673 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-089 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0427 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502792 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2018-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_935cc07c64f3115f589ed2f2d77e26f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f64616a781b98d4374d2bb41650840a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6b8c112553c9cb2a0c430de3dfc43dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c620b38e0229d8066c9d81f251b4ec7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_037e98181e3369eb3667f029e7ecb79d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce89dc96d134035a57c7dd1d27a221e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_908bc762f951ba558705a3e69773bdcb |
| publicationDate | 2020-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3641935-A1 |
| titleOfInvention | Microfluidic analytical device |
| abstract | The invention relates to a device for examining a substance for analyzing nucleic acid, characterized in that it comprises: a first zone comprising a binding site to which the substance to be analyzed is fixed; a first path for causing a first liquid medium to flow to the binding site, thereby allowing the nucleic acid to be progressively pyrophosphorolyzed to its constituent nucleoside triphosphates; a second pathway for removing nucleoside triphosphates from around the fixation site and means for creating in the second path a second medium consisting of aqueous micro-droplets in a water-immiscible support; A micro-droplet handling zone for manipulating the microdroplets by means of optically assisted electro-wetting consisting of: a first composite wall constituted by a first transparent substrate; a first transparent conductive layer; on the substrate having a thickness in the range of 70 to 250 nm; An electromagnetic radiation-activated photoactive layer in the wavelength range of 400-1000 nm on the conductive layer having a thickness in the range of 300-1000 nm and a first dielectric layer on the conductive layer having a thickness of thickness in the range of 120 to 160 nm; A second composite wall consisting of a second substrate; A second conductive layer on the substrate having a thickness in the range of 70 to 250 nm and optionally a second dielectric layer on the conductive layer having a thickness in the range of 120 to 160 nm; Exposed surfaces of the first and second dielectric layers being disposed less than 10 μm apart in order to define a microfluidic space designed to contain the microdroplets; An A / C source for providing a voltage between the first and second composite walls connecting the first and second conductive layers; A source of first electromagnetic radiation having an energy greater than the forbidden band of the photoexcitable layer designed to be incident on the photoactive layer in order to induce corresponding first ephemeral electro-wetting locations on the surface of the first dielectric layer; Means for manipulating the points of incidence of the electromagnetic radiation on the photoactive layer so as to modify the arrangement of the ephemeral electro-wetting sites, thereby creating at least a first electro-wetting path along which the micro-droplets can be made to move; A detection zone disposed downstream of the microparticle handling zone or in one piece with it and a Raman scattering or fluorescence detection system comprising a second source of electromagnetic radiation designed to be incident on the microdroplets in the detection zone and a detector for detecting fluorescence or Raman scattering emitted therefrom. |
| priorityDate | 2017-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 148.