http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3636756-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_20e843db13b2ae4dea539dd10916f294 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2018-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d9c92c433c81d796c67ef727689931f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cea9ea89243056c2ac6268505c5738d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5330b2f39aac52f43445f636accf20ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91ae78c0148bdcaf1dced019a06483f4 |
| publicationDate | 2020-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3636756-A1 |
| titleOfInvention | Method for purifying total mrna from total rna using slfn13 |
| abstract | The present disclosure discloses a method for purifying total mRNA from total RNA with SLFN13, which belongs to the field of biotechnologies. The method for purifying total mRNA from total RNA with SLFN13 according to the present disclosure comprises the following steps of: (1) extracting total RNA; (2) performing enzyme digestion on tRNA and rRNA in the total RNA by using SLFN13; and (3) after the enzyme digestion, directly heating at 70°C for 15 min to inactivate the enzyme, so as to obtain the purified total mRNA. The present disclosure breaks the traditional concepts of RNA purification, and introduces a specific RNA endonuclease to remove tRNA and rRNA, which are to be excluded from the total RNA in order to obtain pure mRNA, by enzyme digestion. The method of the present disclosure is simple, convenient and highly-efficient. The present disclosure has advantages such as low cost, easy availability, simple and convenient operation, time saving, good purification effect, and being contributive to ensure the stability of mRNA. |
| priorityDate | 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.