| abstract |
The present invention relates to an in vitro method for predicting the sex in fish comprising (a) Determining the methylation levels at the CpG sites of (i) the dmrt1 gene promoter within the DNA region comprising the sequence SEQ ID NO: 1, and (ii) the cyp19a1a gene promoter within the DNA region comprising the sequence SEQ ID NO: 2 , and (b) determining the probability of being a female, F P , wherein if F P > 0.5 the fish is a female and if F P < 0.5 the fish is a male. |